ways to protect the column from damaging

sorry for the super late post!

Since the column of the HPLC is very expensive, we can have to do things to maximize the length of its life:)

Here are the things to do:
- during shutdown, always flush the column with ultrapure water to remove all the salts present from the buffer. If this is not done, salts will precipitate and cause clogging of column.
- after which, flush the column with methanol to make the environment unsuitable for microbial growth.
- the ultrapure water used should be changes every 3 days. Since when the ultrapure water is exposed to the air, the airborne bacteria/ bacterial nutrients will enter and multiply in the water. If this contaminated water is used to run experiment, the bacteria will enter and cause column clogging.
- sample injected into the column should always filter will a syringe filter to remove any contaminates.
- reagents used in HPLC should be of HPLC grade

eriko wong
0700477C