hello pple.
it's my turn again.
so i'm going to write about a test done in micro lab.
Wampole™ TOX A/B QUIK CHEK®
this test is a rapid immunoassay for detecting Clostridium difficile toxins A and B.
specimen: fecal specimen
suspected disease: C. difficile disease
(before proceeding with this test, we have to bring all reagents and devices to room temperature to ensure accurate results.)
Required Reagents:
- Diluent
- Congugate
- Wash Buffer
- Substrate
ASSAY PROTOCOL
1. Dilute specimen
using a small glass test tube, add 25μL of specimen to 500μL Diluent + 1 drop of Congugate. mix well.
2. Add sample
transfer 400μL of diluted sample-conjugate mixture to sample well. incubate for 15minutes.
3. Wash Membrane Device
add 300μL Wash Buffer to Reaction window
4. Add Substrate reagent
2 drops to Reaction window. incubate and read results 10 minutes later.
5. Interpretation
read from Reaction window.
two lines -> positive
1 control line -> negative
* invalid test if there is no control line. repeat test pls.
* a positive result may be interpreted at any time during the 10minute interpretation but cannot be ruled as neg or invalid until after 10 minutes.
wendy ong/0701158h
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What is the clinical significance of detecting clostridium difficile, is cultural method available to detect it?
ReplyDeleteYong Herng
0702243G
can the results vary if not read immediately after 10 minutes? For this test done in my lab is similar but my test kit states that it must be read within 30 seconds after the last incubation. There have also been cases where it changes colour if left longer.
ReplyDeleteMuna
0703791D
Clostridium difficile is an opportunistic nosocomial pathogen that affects mainly the young, elderly and immunocompromised individuals. It is a Gram-positive, spore-forming rod that is strictly anaerobic. It causes antibiotic-associated diarrhoea (AAD) and in severe cases, pseudomembraneous colitis. Infection and disease cases have risen over the years especially in the USA due to it's antibiotic resistance. Treatment with antibiotic exacerbates the disease.
ReplyDeletesorry for the extremely late reply. and thanks for the information, dr lee.
ReplyDeletemany patients develop gastrointestinal problems after treatment with antibiotics. thus, altering the normal flora causing this opportunistic anaerobic bacterium to grow in the intestine.
yh: there are selective agars such as C. difficile agar which can be used to detect this organism by cultural method.
hi muna: hmm in our lab, we set the alarm, after 10 mins, read result then tthrow the kit away. so i haven seen any one with colour change. sorry i cannot really answer your question. (;
sorry again for the late reply!
thanks c: