For hematology section, tests such as full blood count (FBC), erythrocyte sedimentation rate ESR), peripheral blood films (PBF), and retic count are done. there are also malaria tests and dengue panel.
For FBC. the tests are done in Celldyn Ruby analyzer which can be done under open or close module. the samples which arrive in EDTA tubes are firstly check for clots. Clotted blood are rejected and staffs will inform the staff nurse in charge. The test will also be cancelled. If the blood is not clotted, the volume of the blood that is above the cap can be done in close module while anything below the cap needs to be done under open module. The results of the tests that are within the range are aoto-validated and the samples can be filled. Some of the auto-validated samples are kept and the results are printed. the samples is then smeared, stained and observed under microscope. This is to ensure that the results between the analyzer and microscopy correlates. The results which are out of range are validated by the staffs.
Every morning and afternoon, the staffs will select one sample whicj the level of the blood is half of that of the tube. The staffs will do correlation whereby the sample is tested on the two analyzer on open module. The results are recorded to ensure that both analyzers give the same results (estimated).
Nyzah
0702888i
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Hi. erm what is actually close and open modules? and the difference in function bwtween both of them?
ReplyDeletejennifer
Hey Nyzah =)
ReplyDeleteHow is auto-validation carried out exactly ?
Thanks =)
Ng Tze Yang Justin
0703747F
Jennifer;
ReplyDeletethe close module is able to load more than one sample at one go as it uses the test tube rack.
the open module can only load one sample at a time.
Nyzah
Justin;
ReplyDeleteThe machine will automatically validate the reuslt.
Nyzah
Hi Nyzah,
ReplyDeletedo you perform the smearing and staining straight after the samples are analyzed or how long can they be stored?
Yvonee
0703189A
When you do smearing and staining, do you do it so that you can count the cells manually or just for observation?
ReplyDeleteWhat do you mean by close and open modules, other than what you have answered to jennifer.
Alvin
Yvonne;
ReplyDeletestaining and smearing is perform right after the sample is analysed. they are not stored and will be thrown straight away after observed under microscope
Alvin;
for staining and smearing of auto validated samples is just to count the cells manually and correlates with the machine results.
the difference between close and open module is just how many sample u can load in at one point of time.
nyzah
Hey nyzah,
ReplyDeleteYou mentioned about the clotting of blood samples? Why would the blood clot when it is already put into an anticoagulant tube?
Felicia
0703345I
hello nyzah,
ReplyDeletein your lab, do you make slides for every specimens or only those with abnormal results for the FBC?
thks,
wendy
0701158h