Human Pancreatic Polypeptide (PP)

Human pancreatic polypeptide (PP) is a polypeptide hormone secreted by the pancreatic polypeptide (PP) cells of the islets of Langerhans in the endocrine portion of the pancreas. It consists of a chain of 36 amino acids.

The function of PP is to self regulate the pancreas secretion activities (endocrine and exocrine) and it also has effects on hepatic glycogen levels and gastrointestinal secretions. It is triggered in humans by protein-rich meals, fasting, exercise, and acute hypoglycemia and is inhibited by somatostatin and intravenous glucose.

The purpose of this test is for the non-radioactive quantitation of human pancreatic polypeptide in human serum and plasma. The principle of this test is by sandwich enzyme-linked immunosorbant assay (ELISA) based sequentially on:

1. Capture of human PP molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-PP antibody.

2. Wash away of unbound materials from samples.

3. Binding of second biotinylated anti-PP polyclonal Ab to the captured molecules.

4. Wash away of unbound materials from samples.

5. Conjugation of horseradish peroxidase to the immobilized biotinylated Ab.

6. Wash away of free enzyme conjugates.

7. Quantification of immobilized Ab-enzyme conjugates by monitoring horseradish peroxidase activities in the presence of substrate 3, 3’, 5, 5’-tetramethylbenzidine.

The enzyme activity is measured spectrophotometrically at 450nm with 630nm background correction. Increase in absorbency is directly proportional to the amount of captured human PP in the unknown sample.

A standard curve is generated by plotting the absorbance versus the respective reference standards of known concentrations of human PP. The concentration of human PP in the samples is then determined directly from this standard curve.

Range of this assay: 12.3pg/mL to 3000pg/mL human PP (50uL sample size)

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