Monday, July 6, 2009

Third Week - Rabbit's blood collection

Done by: Eriko
Section: Haematology

YohOoo! So, last week I did blood collection from rabbits.

Firstly, to prevent the rabbit from moving as much, it is wrapped around with towel with only the head and ear exposing. Then, its ear is cleaned with alcohol swab to disinfect that area for blood collection. A needle is then injected into the central auricular artery. When this step is performing and the rabbit moves, the needle is removed immediately and the processes have to be repeated. Sometimes, we have to massage the other ear to distract the rabbits so as they don't shake/move when we are injecting with the needle.

Then, a syringe is connected to the needle to draw the blood. When the desired amount of blood is drawn, gauze is placed on top of the injected area to apply pressure so as to stop the bleeding. Blood is then injected to EDTA tube and inverted a few times to prevent blood clot.

However, during the blood collection, the blood will always clot. Thus, a pre-rinsed anti-coagulant syringe is preferred to be used in this case.

Here's a picture of blood collection from rabbit to help you picture it better:)


Retrieved on 6 July, 2009 from website media.wiley.com

That's it, guys!

Good luck and enjoy your SIP!~~~~

xo
Posted by at

19 comments:

  1. TG01-Group 2July 6, 2009 at 2:11 PM

    eriko,
    Why is the blood drawn from the central auricular artery and not from other parts.

    Thank you.
    Stanley

    ReplyDelete
  2. BordetellasJuly 6, 2009 at 2:13 PM

    Hi! Stanley!

    I think it is because the central auricular artery is the largest artery there and the easiest to see:)

    See you SoOOoONN~~

    eriko

    ReplyDelete
  3. TG02 - Group 8July 6, 2009 at 5:23 PM

    Hi Eriko,

    why is it that the process has to be repeated if the rabbit moves?

    Yvonee, Group 8

    ReplyDelete
  4. AnonymousJuly 6, 2009 at 9:22 PM

    Heyhey Eriko,

    How much blood do you actually obtain from the rabbit? Why do you not put the rabbit on anaesthesia before collecting the blood so that it does not move?

    Loves,
    Renee
    TG02
    0703634F

    ReplyDelete
  5. fpath-marmarJuly 7, 2009 at 3:24 PM

    How much EDTA is required to prevent blood from clotting? Dr. Khin

    ReplyDelete
  6. BordetellasJuly 8, 2009 at 1:57 PM

    Hi! Yvonee!

    It is because when the rabbit moves, the needle is removed immediately, so then, you are supposed to make sure it is wrapped properly and then have to wipe the area with alcohol swab etc etc..

    see you!

    eriko

    ReplyDelete
  7. BordetellasJuly 8, 2009 at 2:00 PM

    Hi! Renee!

    From the central auricular artery, we can only get a very small amount of blood. I would say it's about 3ml..

    I'm not sure why too but I think it's not essential as rabbits are usually quite tame and they don't really move when the person performing is very experienced.. But this week, we did obtain blood from the rabbit's heart, which we did inject anaesthesia.

    Hope that helps! see you!

    eriko

    ReplyDelete
  8. BordetellasJuly 8, 2009 at 2:02 PM

    Hi Dr. Khin!

    As we are using the EDTA tube, which is 7.2mg of K2EDTA to 4ml of blood. So the amount of K2EDTA requried to prevent blood clotting is 1.8mg/ml:)

    See you!

    eriko

    ReplyDelete
  9. kenneth --grp 10July 8, 2009 at 8:24 PM

    hello eriko,
    what you mean by pre-rinsed anti-coagulant syringe ?
    thanks

    ReplyDelete
  10. emadtechsJuly 11, 2009 at 7:57 PM

    Hey Eriko,

    What happens when a hematoma forms as a result of blood drawing from the rabbit ? What can be done to prevent it ?

    Thanks,

    Ng Tze Yang Justin
    0703747F

    ReplyDelete
  11. Yeo Tiong HanJuly 12, 2009 at 2:20 AM

    Hey,

    You use a prerinsed syringe that contains anticoagulant.After that, you release the drawn blood to EDTA tubes.

    May i ask what is the rationale of doing it? Is it possible to put it into plain tubes. The Syringe already mixed the blood with anti coagulant already.

    Cheers
    Tiong Han
    0703762E

    ReplyDelete
  12. BordetellasJuly 13, 2009 at 10:25 PM

    Hi, Kenneth!

    hmm, it just means the syringe has to be rinsed/washed with anticoagulant first before it is used to draw the blood.

    For my experiment, I draw some dissolved EDTA into the syringe and pull it to its end and then force all out.

    Hope that helps~

    eriko

    ReplyDelete
  13. BordetellasJuly 13, 2009 at 10:27 PM

    Hi Justin!

    If hematoma is formed, the collection of blood will be stop and needle will then be withdrawn. Afterwhich, a gauze is used to pressed onto that particular area to stop bleeding.

    The use of prerinsed EDTA syringe is to prevent this from happening:)

    Hope that helps!

    eriko

    ReplyDelete
  14. BordetellasJuly 13, 2009 at 10:34 PM

    Hi Tiong Han!

    The rationale of doing it is to prevent blood from clotting during collection.

    As for the second question, the syringe is only 'coated' with some EDTA and this can only help to prevent the blood from clotting for a short time. So after collection, we have to transfer to an EDTA tube and inverting a few times to ensure a thorough mixing.

    Hope that helps!

    eriko

    ReplyDelete
  15. The Madtechs Gp09 - TG02July 16, 2009 at 1:46 PM

    hi eriko,

    just to ask, besides the central auricular artery at the ear, is there any other arteries or body parts that the blood can be drawn out from?

    many thanks,
    Gwendolynn
    0703953J
    TG02

    ReplyDelete
  16. UnknownJuly 20, 2009 at 11:25 PM

    Hi Eriko, :)

    I was wondering why are you collecting blood from rabbits? Is it related to your project or are you doing some evaluation?

    Rachel :)

    ReplyDelete
  17. emadtechsJuly 22, 2009 at 11:20 PM

    Hey Eriko,

    what happens if you accidentally inject the needle into other arteries except the central auricle artery? Do you still proceed with the blood taking or you repeat the process all over again? Thanx!

    Hui Juan
    0702012F

    ReplyDelete
  18. BordetellasJuly 25, 2009 at 1:46 PM

    @gwen - Hii!! yes there are. From what I know, blood can be directly taken from the cardiac or from a artery around the joint of the arm.

    @rachel - Hi!! oh, ya. It is related to my project:)

    @hui juan - Hi!! Hmm.. I think that we can proceed on because we have taken blood from other artery on the ear:)

    eriko (0700477C)

    ReplyDelete
  19. karlalaJuly 15, 2011 at 11:45 PM

    hi, may i know the weight of your rabbit? or how old are they?

    ReplyDelete

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