hii it's my turn now !
so, i'll be writing about the department i was allocated last week, urinalysis. before i start on the processes of tests done by this bench, there are some preparations and QCs to mention briefly. this is also what we usually do to start off the exciting day. after rollcall, i'll be preparing the 0.1% hypochlorite. pretty easy as they're in the tablet form. only thing to note is- how many tablets to how much DI water. for QC, we have to do it for the osmometer, refractometer, ABG analyzer and the dipstix analyzer. materials used for these QCs are mainly DI water, saline and commercial solution.
okay, so now i'll begin on the tests/skills i've learnt.
1. urine FEME
this test includes both dipstix and microscopic examination (ME). we should be familiar with the procedures for this test thus i'll summarize what i've learnt. there is a correlation between the dipstix result and the ME.
if there is a presence of protein, there could be casts present under the ME. similarly, positive nitrate shows presence of microorganism, positive esterase shows presence of WBCs.
2. rapid plasma reagin (RPR) card test
to test for syphilis using the patient's serum. we use a kit, Omega Diagnostics Immutrep RPR to carry out this test with only one drop of serum. there is a positive and negative control to compare with the samples. positive results will show agglutination.
3. dengue panel
this test is also carried out by using a commercial kit, SD Dengue Duo, a one step dengue NS1 Ag and IgG/IgM test. time taken is around 15 minutes.
4. arterial blood gas (ABG)
ABG!! that's what the order entry person shouts after they have ordered this test. reason being, it must be done asap to obtain an accurate result as this test measures the pH and the partial pressure of oxygen, carbon dioxide and also, bicarbonate concentration of the blood sample. the analyzer used is called Roche OMNI C. just have to poke the needle into the syringe and the analyzer produce the results. so, it is pretty simple. however, most of the time the med tech will be doing this as their pass are needed to operate this analyzer. i've tried it couple of times tho. ;)
5. HCG test
there is a test kit for this too and patient's serum is used. altho urine can also be loaded on the kit, serum is preferred as it gives a more accurate result. it's a straightforward test with either a +/- shown after 15 minutes.
ps i have photos to upload but dontknow how :s will add in after figuring out.
enjoy weekends,
wendyong
Subscribe to:
Post Comments (Atom)
Hey Wendy!
ReplyDeleteSherman here ^^
Ask you about the refractometer.
What is the principle of the equipment?
I see here see there, I dunno which name goes to which machine @.@ hahaha!
For the QC, you used saline, DI water and commercial solution.
What are the perimeters you have to observe to ensure that the refractometer pass the QC, like what results must you see? Izit likecolour change, agglutination, numerical figures etc.
THANKS! =DD
You mention that rapid plasma reagin (RPR) card test, dengue panel and HCG test all uses commercial test kit.
ReplyDeleteSo how does each commercial test kit works?
For HCG test, why is serum the more preferred choice?
Alvin
hi wendy!
ReplyDeletefor dengue panel, what type of specimens do you use? EDTA or serum?
nyzah
hiii pple.
ReplyDeletesherman:
refractometer is use to measure the specific gravity of a liquid. you know in the dipstix there's also this component. so, in my lab we use it for fluids such as CSF, pleural fluid, knee fluid etc. the range is from 1.000-1.040. basically, you have to put 2 drops of sample onto the measuring plate and close the plate cover, look through the eyepiece lens where the scale is magnified to read the result. :D
alvin:
for RPR, there will be binding btw lecithin/choesterol/cardiolipin in the reagent and the reagin antibodies in the sample and the results can be seen in the form of black clumps (+ve) due to the IMMUTREPVDRL Ag which contains carbon particles.
dengue: basically, how it works is that the dengue IgGs and IgMs in the specimen will react with the antigen forming the complex of antibodies-antigen. this complex will migrate thru capillary action until it is captured by the relevant anti-human IgG/IgM immobolized in the 2 test lines and generate a coloured line.
hcg: so after loading the serum, it will migrate thru the membrane and mobilises the anti-hCG monoclonal antibody-colloid. if there's hCG, it will form a complex with the antibody-colloid. this complex will migrate and then captured by the immobilised anti-hCG polyclonal antibody in the result window, showing the + sign.
serum is preferred because there will be a higher concentration so the test will take a shorter time.
nyzah!:
EDTA? err, we use serumm ah. :D
Wendy, please focus only on one test. Follow what is instructed in the blog induction. Tks.
ReplyDeleteHi,
ReplyDeleteI have visited your post and found some information which is very important for me.
So, please keep posting.Caspase Assay Kit